The response of human induced pluripotent stem cells to cyclic temperature changes explored by BIO-AFM

Abstract Human induced pluripotent stem cells (hiPSCs) are highly sensitive to extrinsic physical and biochemical signals from their extracellular microenvironments. In this study, we analyzed the effect of cyclic temperature changes on hiPSCs behaviors, especially by means scanning force microscopy (BIO-AFM). The alternation in cellular mechanics, as well secretion pattern deposition matrix (ECM) protein were evaluated. arrangement actin cytoskeleton changed with variation temperature. rearranged architecture led subsequent cell mechanics (Young's modulus hiPSCs). With exposure cold stimuli, an increase average surface roughness (Ra) mean square (RMS) was detected. This observation might be at least part due upregulated Laminin α5 during repeated temporary cooling. expression markers, NANOG SOX2, not impaired hiPSCs, when exposed stimuli for 24 h. Our findings provide insight into hiPSC which may contribute a better understanding application locally controlled therapeutic hypothermia. Graphic abstract changes, 37 10 °C, rapidly increased mechanical strength human-induced (hiPSCs), could explained re-arrangement cytoskeletons. capacity remodel also altered cooling, they exhibit enhanced ability physically remodulate secrete ECM components.